Orochimaru agonizes in pain at his hideout, now requiring level-ten medications just to sustain his body. Even with excruciating pain Orochimaru is preparing for his transference ritual, and Kabuto can feel Orochimaru's further potential. Meanwhile Sasuke also senses the time is coming when he will have to face his mentor...
Orochimaru sheds his old body and transforms into a white serpent. As he attacks Sasuke to obtain his body, Orochimaru remembers the time he found the cast off skin of a white snake in front of his parents' grave. Driven by his desire for the Sharingan, Orochimaru finally robs Sasuke of his freedom...
Sasuke has defeated Orochimaru. He then frees Suigetsu, a man trapped inside a water tank, and tells the man to join him. But Suigetsu does not immediately take up Sasuke's offer…
Sasuke and Suigetsu visit the southern hideout to recruit their next member, Karin. Suigetsu is surprised at the hideout's low security level but is told that no prisoners can escape thanks to Karin's abilities. What is Karin's power that allows her to maintain order and an iron defense?
Sasuke and his team head for the northern hideout to recruit their next member, Jugo. On the way, Karin explains that the northern hideout is a site for conducting horrific human experiments. A ninja with abnormal homicidal impulses, Jugo is the sole carrier of the enzyme necessary to create Orochimaru's curse mark...
Slipping past the riot, Sasuke arrives at Jugo's cell. But Jugo refuses to be released, even going as far as to say he rather die a lonely death than to leave the hideout.
"More than ten years ago, the rule of the Five Great Shinobi Nations was destabilized, throwing the world into a conflict later to be known as the Third Great Ninja War. The future Fourth Hokage Minato Namikaze assigns young Kakashi, Obito of the Uchiha clan, and Rin, a medical ninja, on a mission to Kannabi Bridge..."
Obito ignores Kakashi's order to continue with the mission and leaves to save Rin, who was kidnapped by the Rock ninja. But the enemies detect Obito's arrival and defeat him. Kakashi arrives at the last minute and saves Obito…
After hunting down the Four Tails, Itachi and Kisame are summoned by the leader of the Akatsuki. The leader announces the death of Orochimaru to the gathered members and the possibility of his killer, Sasuke, setting his sights on the Akatsuki organization....
Under Kakashi's command, the Leaf ninja set off to find Itachi. Using Kakashi's ninja hounds, they gather as much information as they can on Itachi. Meanwhile, the members of the Hebi also scatter to look for information on Itachi. Led by the ninja hound tracking Sasuke's scent, Sakura arrives in town and draws near to Karin.
Upon encountering one another, Sasuke and Deidara engage in battle. After exchanging a few blows to test Sasuke's strength, Deidara conjures up his specialty clay dragon and wages long-range attacks against Sasuke from high above. With Tobi's backup support, the battle seems to be going to Deidara.
Determined to destroy the Sharingan to rid himself of the taste of defeat he received from Itachi, Deidara unleashes his biggest attack, the C4 Karura. Created to kill Itachi, this fierce weapon is capable of destroying living beings from the inside using millions of nano-sized bombs. Can Sasuke escape this attack fueled by Deidara's determination to destroy the Sharingan?
Sasuke goes missing after being caught by Deidara's suicidal bombing. Sensing his disappearance, Jugo, Karin, and Suigetsu race to Sasuke's last location, where the three Hebi members discover...?
Leaf ninja, she notifies the others and the Hebi members quickly leave the inn. To allow Sasuke to focus on his coming battle with Itachi, Karin comes up with a plan to keep the Leaf ninja occupied.
During the reign of the Second Hokage, the young Jiraiya, Orochimaru, and Tsunade are assigned to Hiruzen Sarutobi's platoon as Genins. Fascinated by Sarutobi's summoning jutsu, Jiraiya immerses himself in mastering the transportation technique. While training, Jiraiya accidentally transports himself to Mount Myoboku, opening the path to fulfill his destiny.
Jiraiya's journey to seek out a student is postponed due to the outbreak of the Third Great Ninja War. Jiraiya joins Tsunade and Orochimaru to battle the Village Hidden in the Rain. Following the battle, the three ninja are named the "Legendary Sannin," a title given to them by the enemy Hanzo of the Great Salamander himself.
Jiraiya successfully infiltrates the Hidden Rain Village by hiding inside the toad Gamagoro. He sets out to gather information but finds no indications of a civil war occurring within the village.
Foreseeing a possible battle, Jiraiya takes precautionary measures by releasing the scroll toad that commands the key to Naruto's Eight-Signed
Jiraiya battles to defeat his former student Pain, who is now driven by his twisted ideals as an Akatsuki member.
Jiraiya completes his Sage Mode transformation to battle the three Pains.
Having lost his arm, Jiraiya is outmatched by the six Pains now appearing before him. Of the six, the one called Tendo is revealed to be Jiraiya's former student Yahiko. Jiraiya is still puzzled by the Rinnegan's true powers, but before he can gather his thoughts, he is forced back into battle.
Sasuke arrives at one of the Akatsuki hideouts, having followed in Itachi's footsteps. When Sasuke is about to enter the hideout alone, his older brother Itachi finally appears before him…
While Itachi waits for Sasuke in the Uchiha hideout, he recalls the past incident that led to his defection from the Leaf Village. When Sasuke arrives, the fated battle begins between the two brothers.
Following their fierce genjutsu battle with the Sharingan, Itachi explains the secret behind the most powerful ocular jutsu, the Mangekyo Sharingan, and the history of the Uchiha clan…
Sasuke loses his left eye to Itachi, but it turns out to be a genjutsu created by the Mangekyo Sharingan. Breaking free of the Tsukuyomi by force, Sasuke makes a furious comeback against the injured Itachi.
At the climax of the battle, both Itachi and Sasuke run out of chakra. However, Sasuke reveals that his actions up until now were only a preparation for his final jutsu.
Naruto and his comrades find themselves held up by Tobi, whose strange jutsu is more than just a simple genjutsu.
In the Akatsuki hideout Sasuke comes face to face with Madara. Announcing himself as Itachi's accomplice from that fateful night, Madara goes on to tell Sasuke the truth about Itachi that he could never have known.
The Uchiha clan was plotting a coup d'etat. The story as told by Madara leaves Sasuke at a loss for words. Because of his gifted talent, Itachi was forced to suffer, torn between his loyalties to the Leaf Village and the Uchiha clan.
Flame from the Amaterasu still remains on the battlefield as Naruto and his teammates continue to search for Sasuke. But despite the search team's best efforts, they cannot find any trace of him.
The Taka split up with the Akatsuki to hunt down the remaining Tailed Beast. At the Land of Lightning they go up against Killer Bee, whose attack utilizing Lightning Style and seven blades proves to be more than a match for them.